Representative images of microglial immunostaining in C3H/HePas wild-type (WT) and C3H/HeJ Toll-like receptor (TLR)4 mutant mice 1 week after sciatic nerve unilateral axotomy. (A,C) Unlesioned side and (B,D) lesioned side of the WT and TLR4 mutant mice. Note the higher Iba1 expression on the surface of the axotomized neurons in both groups, with no differences between groups. The dashed circle indicates the motor nucleus containing the alpha motor neurons. (E) Graph representing the ipsilateral:contralateral ratio of the integrated density of pixels (P>0.05). Scale bar: 50 μm.