Diapocynin inhibits activation of microglia and astrocytes in the substantia nigra (SN) of MPTP-treated mice. (A) 3,3'-Diaminobenzidine (DAB) immunostaining of Iba-1 in SN. (B) Representative Western blots illustrating the expression of Iba-1 in SN. (C) Bar graph showing quantitative densitometric analysis of Iba-1/β-actin ratio ± SEM in SN of 6 mice per group. (D) DAB immunostaining of glial fibrillary acidic protein (GFAP) in SN region of ventral midbrain. (E) Representative Western blots illustrating the expression of GFAP in SN. (F) Bar graph showing mean Western blot GFAP/β-actin ratios ± SEM in SN of 6 mice per group. Images were captured at 30× magnification. **
P <0.01 vs the control group; *
P <0.05 vs the MPTP group.