Diapocynin attenuates NADPH oxidase mediated inflammatory responses in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of gp91phox (membrane-bound subunit of NADPH oxidase) in SN. (B) Bar graph showing mean Western blot gp91phox/β-actin ratios ± SEM in SN of 6 mice per group. (C) SN tissue sections were double labeled for gp91phox and Iba-1. Images were captured at 20× and 60× (insets) magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of Iba-1 and gp91phox. ***
P <0.001 vs the control group; *
P <0.05 vs the MPTP group.