Figure 3

More severe axonal loss/damage in the white matter of Prnp ^-/- mice. Sections of ventral spinal cord revealed by Bielschowsky's silver staining; (A) Prnp ^+/+ EAE-induced mouse; (B) Prnp ^-/- EAE-induced mouse and (C) non-EAE PBS/CFA control. (D) and (E) focus into the white matter of ventral spinal cord from a Prnp ^+/+ EAE mouse and a Prnp ^-/- EAE mouse, respectively. (F) higher magnification in the damaged white matter of a Prnp ^-/- EAE mouse showing typical axonal bulb formation. Scale bars: A-C: 200 μm, D-E: 30 μm, F: 15 μm. (G) quantitative evaluation of axonal damage in Prnp ^+/+ versus Prnp ^-/- EAE-induced mice, and wild type PBS controls (n = 3/group). *: p < 0.05 vs. PBS controls (Dunn's post test analysis).