Figure 8

Representative immunostaininings for PMCA2 and GFAP in the cerebellum of chimeric mice with EAE. Double indirect immunofluorescence for PMCA2 and GFAP followed by DAPI staining was performed on cerebellar sections from [Prnp^-/- > Prnp^+/+] or [Prnp^+/+ > Prnp^-/-] mice with EAE. (A-B) PMCA2 immunofluorescence and (C-D) GFAP/DAPI stainings in corresponding section of the grey matter. (E-F) GFAP/DAPI stainings in the white matter (cerebellar peduncle). ml, molecular layer of the grey matter; pl, Purkinje layer of the grey matter; gl, granular layer of the grey matter; v, blood vessel; wm, white matter. Scale bar, 100 μm.