The microglia marker Iba1 is enriched in podonuts and podosomes. All panels show high-resolution, deconvolved images. (A). Representative microglia showing strong Iba1 immunoreactivity (red) in the lamellar ring. Vimentin immunoreactivity (green) is also shown and nuclei are labeled with DAPI (blue). Scale bar, 20 μm. (B) A microglial cell stained for Iba1 (red) and the podosome core marker, F-actin (phalloidin; green). Scale bar, 5 μm. (C) Merged and color-separated images immunostained for Iba1 (green) and the podosome ring marker, talin (red). Scale bar, 5 μm. C’: High-magnification images of talin and Iba1 in individual podosomes. Scale bar, 1 μm.