The effects of morphine pre
treatment on IL
regulation of the MOR in U87 MG cells
. U87 MG cells were pre-treated with either vehicle (control) or morphine (100 nM) for 24 h. Following pre-treatment, the medium was aspirated and fresh cell culture medium containing either vehicle or IL-1β (20 ng/mL) was added and the cells were incubated for 12 h. Absolute quantitative real time RT-PCR (AQ-rt-RT-PCR) was used to determine expression of the MOR and GAPDH. Levels of MOR were normalized to GAPDH. Data are the mean ± SE. A Student’s t-test was used to determine significance. *P <0.05 compared to the control; #
P <0.05 compared to IL-1β (alone); ^P <0.001 compared to the control.