α-SYN internalization in WT and FcγR
mouse primary microglia. (A-H) 72 h after the treatment of aggregated human α-SYN, WT and FcγR−/− mouse primary microglia were immunostained for human α-SYN (Green) and the microglial marker CD45 (Red). Condensed α-SYN was observed in the WT microglia. In FcγR−/− microglia, there was still uptake of aggregated human α-SYN but the pattern of intracellular α-SYN was quite different, with diffuse labeling of small puncta throughout the cytoplasm. Scale bars: panel A, B, E, F bar=50 μm; panel C, D, G, H bar=20 μm. Image 1 in Panels D and H are confocal images of WT and FcγR−/− mouse primary microglia treated with aggregated human α-SYN, respectively; 2 and 3 in Panel D and H are transverse and lengthwise images of z-stack series of the indicated cells. (I) 24 h after the treatment of aggregated human α-SYN, WT and FcγR−/− mouse primary microglia were immunostained for human α-SYN (Green) and autophagosomal marker LC3B (Red). α-SYN co-localized with LC3B in WT but not FcγR−/− microglia. Phase contrast images show the morphology of the cell. Scale bar=10 μm.