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Table 2 Data analysis showed alterations in the expression of macrophage-associated, regulatory and proinflammatory genes in cortical regions of postmenopausal women, indicating overlapping changes with the rat results

From: Menopause leads to elevated expression of macrophage-associated genes in the aging frontal cortex: rat and human studies identify strikingly similar changes

  

Microarray analysis

Real-time PCR

Symbol

Probeset

FC (PG)

P * (P G)

FC (SG)

P * (S G)

RQ (age+OVX)

P (age+OVX)

Macrophage-associated genes

Phagocytic and scavenger receptors

CD11b b

205786_s_at

1.341

0.918

1.145

0.832

2.778

< 0.001

CD18 b

1555349_a_at

2.808 a

0.697

2.331 a

0.629

2.164

< 0.001

FCGR2b

210889_s_at

1.020

1.000

1.228

0.746

2.488

< 0.001

CD36

228766_at

0.670 a

0.942

0.812

0.793

0.440

< 0.001

RAGE

217046_s_at

1.000

1.000

0.991

0.960

1.066

 

Recognition receptors

CD14

201743_at

5.134 a

0.697

2.817 a

0.629

1.174

 

CD40 b

205153_s_at

1.025

0.877

0.967

0.881

1.481

0.054

CD45 b

212588_at

1.903 a

0.918

1.152

0.938

1.799

< 0.001

CD86 b

205685_at

1.466

0.697

1.094

0.709

1.782

< 0.001

TLR2

204924_at

1.832 a

0.781

1.035

1.000

1.015

 

TLR4

221060_s_at

1.152

1.000

1.634 a

0.629

1.512

< 0.001

TLR9

223903_at

  

1.182

0.629

1.907

0.008

CD74 b

1567628_at

2.232 a

0.697

1.631 a

0.689

2.255

0.047

Signaling

NOS2

212531_at

0.965

1.000

1.002

1.000

1.041

 

IRF7

208436_s_at

1.047

1.000

1.341

0.629

4.748

< 0.001

IRF9

203882_at

1.105

1.000

1.242

0.629

1.746

< 0.001

Regulatory genes for microglia reactivity

Microglial receptors

CD200R

1552875_a_at

0.990

0.942

0.990

0.763

0.722

< 0.001

CX3CR1

1568934_at

0.557 a

0.967

0.960

1.000

1.341

0.005

Neuronal ligand

CD200

209582_s_at

0.886

1.000

0.497 a

0.629

0.829

0.019

SIRPA

202897_at

0.799

0.877

0.908

0.750

0.987

 

CX3CL1

823_at

0.462 a

0.697

0.575 a

0.629

0.754

< 0.001

Complement and cytokine genes

Complement

C1q

202953_at

4.364 a

0.697

2.319 a

0.629

1.654

< 0.001

C1-INH

200986_at

1.419

0.877

1.723 a

0.629

1.553

0.009

C3

217767_at

1.577 a

0.942

1.246

0.842

5.126

< 0.001

Chemokine and cytokines

CCL2

216598_s_at

1.976 a

0.942

2.492 a

0.629

2.114

0.014

IL1b

205067_at

0.669

0.984

0.743

0.763

1.238

 

IL6

205207_at

0.594 a

0.907

0.748

0.709

0.728

 

TGFb1

203085_s_at

1.812 a

0.781

1.988 a

0.629

1.347

< 0.001

TNF

207113_s_at

    

1.345

 
  1. Differential expression of selected genes in the postcentral and superior frontal gyrus of pre- and postmenopausal women. Fold change (FC) corresponds to the ratio of the older and younger group mean expressions for a given gene. aFC > 1.5; P* false discovery rate-adjusted raw P-value; RQ(age+OVX) values correspond to the ratio of mRNA expression in middle-aged OVX rats compared to young ones; bconventional microglial marker genes are in bold.
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Journal of Neuroinflammation

ISSN: 1742-2094