Skip to main content

Table 2 Data analysis showed alterations in the expression of macrophage-associated, regulatory and proinflammatory genes in cortical regions of postmenopausal women, indicating overlapping changes with the rat results

From: Menopause leads to elevated expression of macrophage-associated genes in the aging frontal cortex: rat and human studies identify strikingly similar changes

   Microarray analysis Real-time PCR
Symbol Probeset FC (PG) P * (P G) FC (SG) P * (S G) RQ (age+OVX) P (age+OVX)
Macrophage-associated genes
Phagocytic and scavenger receptors
CD11b b 205786_s_at 1.341 0.918 1.145 0.832 2.778 < 0.001
CD18 b 1555349_a_at 2.808 a 0.697 2.331 a 0.629 2.164 < 0.001
FCGR2b 210889_s_at 1.020 1.000 1.228 0.746 2.488 < 0.001
CD36 228766_at 0.670 a 0.942 0.812 0.793 0.440 < 0.001
RAGE 217046_s_at 1.000 1.000 0.991 0.960 1.066  
Recognition receptors
CD14 201743_at 5.134 a 0.697 2.817 a 0.629 1.174  
CD40 b 205153_s_at 1.025 0.877 0.967 0.881 1.481 0.054
CD45 b 212588_at 1.903 a 0.918 1.152 0.938 1.799 < 0.001
CD86 b 205685_at 1.466 0.697 1.094 0.709 1.782 < 0.001
TLR2 204924_at 1.832 a 0.781 1.035 1.000 1.015  
TLR4 221060_s_at 1.152 1.000 1.634 a 0.629 1.512 < 0.001
TLR9 223903_at    1.182 0.629 1.907 0.008
CD74 b 1567628_at 2.232 a 0.697 1.631 a 0.689 2.255 0.047
Signaling
NOS2 212531_at 0.965 1.000 1.002 1.000 1.041  
IRF7 208436_s_at 1.047 1.000 1.341 0.629 4.748 < 0.001
IRF9 203882_at 1.105 1.000 1.242 0.629 1.746 < 0.001
Regulatory genes for microglia reactivity
Microglial receptors
CD200R 1552875_a_at 0.990 0.942 0.990 0.763 0.722 < 0.001
CX3CR1 1568934_at 0.557 a 0.967 0.960 1.000 1.341 0.005
Neuronal ligand
CD200 209582_s_at 0.886 1.000 0.497 a 0.629 0.829 0.019
SIRPA 202897_at 0.799 0.877 0.908 0.750 0.987  
CX3CL1 823_at 0.462 a 0.697 0.575 a 0.629 0.754 < 0.001
Complement and cytokine genes
Complement
C1q 202953_at 4.364 a 0.697 2.319 a 0.629 1.654 < 0.001
C1-INH 200986_at 1.419 0.877 1.723 a 0.629 1.553 0.009
C3 217767_at 1.577 a 0.942 1.246 0.842 5.126 < 0.001
Chemokine and cytokines
CCL2 216598_s_at 1.976 a 0.942 2.492 a 0.629 2.114 0.014
IL1b 205067_at 0.669 0.984 0.743 0.763 1.238  
IL6 205207_at 0.594 a 0.907 0.748 0.709 0.728  
TGFb1 203085_s_at 1.812 a 0.781 1.988 a 0.629 1.347 < 0.001
TNF 207113_s_at      1.345  
  1. Differential expression of selected genes in the postcentral and superior frontal gyrus of pre- and postmenopausal women. Fold change (FC) corresponds to the ratio of the older and younger group mean expressions for a given gene. aFC > 1.5; P* false discovery rate-adjusted raw P-value; RQ(age+OVX) values correspond to the ratio of mRNA expression in middle-aged OVX rats compared to young ones; bconventional microglial marker genes are in bold.