Figure 3
From: PINK1 positively regulates IL-1β-mediated signaling through Tollip and IRAK1 modulation
Association between PINK1 and Tollip increases upon IL-1β stimulation. (A),(B) 293 IL-1RI cells were transfected for 42 hours with Myc-hPINK1-WT, Myc-hPINK1-KD, or HA-Tollip, alone or in combination, and treated with 10 ng/ml IL-1β for 15 minutes. Cell lysates were subjected to immunoprecipitation (IP) with anti-Myc antibody, followed by immunoblotting with anti-HA antibody. Total cell lysates were also analyzed with anti-Myc and anti-HA antibodies. *IgG heavy chains. Actin served as a loading control. (A) The binding intensity ((Tollip bound/Tollip input)/PINK1 IP) was quantified and normalized to the intensity of IP using Image J software [22]. (B) *P<0.05; n.s., not significant.PINK1, PTEN-induced putative kinase 1; Tollip, Toll-interacting protein.