Expression of breast cancer resistance protein (BCRP) in freshly isolated rat brain microvessels after endothelin-1 treatment of juvenile and adult rats. Rats equivalent to pediatric (P21) and adult (P84) human brain were used. Twenty-four hours after intracerebroventricular (icv) injection of endothelin-1 (ET-1) was used to trigger inflammatory reactions, brain microvessels were isolated, and BCRP expression was assessed by western blot as reported in Methods. Results show no difference in BCRP expression after ET-1 treatment. Immunoblot represents mean ± S.E.M from three batches of brain endothelial microvessels. Statistical comparisons: *P <0.05, **P <0.01 and ***P <0.0001.