Fluorescence intensity demonstrating protein levels for interleukin
-1β, matrix metalloproteinase
9 and tumor necrosis factor
α following subarachnoid hemorrhage
and sham operated animals in the walls of the middle cerebral artery
(MCA). Densitometry analysis showed that there was an early transient increase of IL-6 (A) and IL-1β (B) protein in the walls of the MCA around 6 h which increased over time during 24 to 96 h with a maximum peak at 72 h after SAH. The MMP-9 protein level showed a slight increase at 6 h and a gradual increase during the period of 24 to 96 h with a maximum increase at 48 to 72 h (C). Expression of TNFα protein started during 12 to 24 h, which increased over time until 96 h (D). n = 3, * indicates significant differences as compared to sham-operated rats for each timepoint. *P <0.05, **P <0.01.