(A) Effect of pentamidine on macrophage infiltration in colonic tissue of DSS-treated mice: pentamidine dose-dependently reduced macrophages infiltration. Arrows indicate MAC387 immunopositive macrophages infiltrating colon criptae; (B) Quantification of MAC387 immunopositive macrophages in colon criptae. Data are expressed as mean ± SEM of n = 3 experiments; (C) effect of pentamidine on MPO level in colonic tissue of DSS-treated mice. Results are expressed as mean ± SEM of n = 6 experiments. ***P <0.001 vs. vehicle (saline); °°P <0.01 and °°°P <0.001 vs. DSS. Original magnification 100X. DSS, dextran sodium sulphate; MPO, mieloperoxidase.