(A) Western blot analysis showing S100B protein expression in mucosa (M) and in LMMP preparations. Effect of pentamidine on inflammatory mediators (NO2
-, PGE2, IL-1β, TNF-α) in mucosa (B) and in LMMP (C) in the presence of different proinflammatory stimuli (LPS + DSS or exogenous S100B depending upon the experiments). Results are expressed as mean ± SEM of n = 5 experiments. ***P <0.001 vs. vehicle (saline); °P <0.05, °°P <0.01 and °°°P <0.001 vs. proinflammatory stimulus DSS, dextran sodium sulphate; IL-1β, interleukin-1 beta; LMMP, longitudinal-muscle myenteric plexus; LPS, lipopolysaccharide; NO, nitric oxide; TNF-α, tumor necrosis factor alpha.