Figure 1
Lipoxins and analogues alleviate mechanical allodynia in the CIBP model induced by intra-tibia injection of Walker 256 carcinoma cells (4×10 5 ). (A) The time course of the development of mechanical allodynia after cancer cell inoculation. +++ P<0.001 versus the sham group (by Bonferroni test). (B) Comparisons were also made between the effects of the ATL, LXA4 and LXB4 treatments on rats with CIBP on day 7 after surgery. Data are expressed as means ± SEM. *P<0.05, **P<0.01, ***P<0.001 versus cancer + NS (by Bonferroni test). ## P<0.01, ### P<0.001 versus cancer +ATL (by T-test). (C) Effects of i.t. LXA4, LXB4 and ATL on the mechanical allodynia of naive rats (by Bonferroni test). (D) Anti-allodynia effects of i.t.ATL at different doses on rats with CIBP. On day 11 after surgery, the cancer groups were given different doses of ATL by i.t. injection. Note that the dose of 0.45 nmol showed a better and longer analgesic effect from 2 to 8 hours than the other doses. *P<0.05, **P<0.01, ***P<0.001 versus cancer + NS (by LSD test). # P<0.05 versuscancer + ATL 0.45 nmol (by T-test). (E) Anti-allodynia effects of i.v. ATL at different doses on rats with CIBP. On day 11 after surgery, the three groups were injected i.v. with NS or different doses of ATL (10 nmol/kg or 120 nmol/kg). # P<0.05 versus cancer +ATL 30 nmol/kg (by T-test). Data are expressed as means ± SEM. *P<0.05, **P<0.01, ***P<0.001 versus cancer + NS (by Bonferroni test).ATL, aspirin-triggered-15-epi-lipoxin A4; CIBP, cancer-induced bone pain; i.t., intrathecal; i.v., intravenous; LSD, least-significant difference; LX, lipoxin; NS, normal saline; SEM, standard error of the mean.