Lipoxins and aspirin-triggered lipoxin alleviate bone cancer pain in association with suppressing expression of spinal proinflammatory cytokines

Table 1 Seqences of the forward and reverse primers and PCR conditions used for RT-PCR

Genbank accession numbers	Target gene	Primers	PCR conditions (temperature/time)
			Denature	Anneal	Extend
NM_017008	GAPDH	Foward: 5^′cccttcattgacctcaactac-3^′	94°C/45 seconds	60°C/1 minute	72°C/1 minute
NM_017008	GAPDH	Reverse: 5^′-cttctccatggtggtgaagac-3^′	94°C/45 seconds	60°C/1 minute	72°C/1 minute
NM_031512	IL-1β	Forward: 5^′-atgagagcatccagcttcaaatc-3^′	94°C/45 seconds	58°C/1 minute	72°C/1 minute
NM_031512	IL-1β	Reverse: 5^′-cacactagcaggtcgtcatcatc-^′3	94°C/45 seconds	58°C/1 minute	72°C/1 minute
NM_012589	IL-6	Forward: 5^′-gacaaagccagagtccttca-3^′	94°C/45 seconds	58°C/1 minute	72°C/1 minute
NM_012589	IL-6	Reverse: 5^′-actaggtttgccgagtagac-3^′	94°C/45 seconds	58°C/1 minute	72°C/1 minute
X66539	TNF-α	Forward: 5^′-cgagatgtggaactggcaga-3^′	94°C/45 seconds	58°C/1 minute	72°C/1 minute
		Reverse: 5^′-ctacgggcttgtcactcga-3^′

ISSN: 1742-2094