Figure 4

Gp91 ^phox -p22 ^phox complex is expressed in COS-7 and HEK293 cells. A. Western-blot analysis for gp91^phox, p22^phox, p47^phox and p67^phox in COS-7-gp91^phox-p22^phox and COS-7-NOX2 cells. B. Western-blot analysis for gp91^phox and p22^phox in HEK293 cells. Briefly, 1 × 10⁶ HEK293 cells were seeded into 6-well plates. Flag-gp91^phox and Myc/His-p22^phox expression plasmids were co-transfected into HEK293 cells using Lipofectamine 2000 according the commercial protocol. After 48 hours of expression, cells were collected for western-blot detection.