Inhibitory effects of 4-
-methylhonokiol on the LPS-induced expression of inflammatory proteins. (A) Immunoreactive cells of iNOS antibody were detected in the cortex and hippocampus. 5 μm-thick sections of brains from mice were incubated with anti-iNOS antibodies and the biotinylated secondary antibody. It was then counterstained by hematoxylin. The resulting tissue was viewed with a microscope. (B) The present figure is representative for three different experiments with different animal brains. (C) The expression of iNOS and COX-2 were detected by western blotting using specific antibodies. β-Actin protein was used here as an internal control. The values in the western blot band indicate average density over β-actin from 5 animals. #, Significantly different from control group (p < 0.05). *, Significantly different from LPS-treated group (p < 0.05). Control, saline-treated group. LPS, lipopolysaccharide. MH, 4-O-methylhonokiol.