Effect of 4-
-methylhonokiol on expression of amyloidogenic proteins. (A) Immunoreactive cells of anti-BACE1 antibody were investigated in the cortex and hippocampus. (B) The present figure is representative for three different experiments with different animal brains. (C) The expression of APP, C99 and BACE1 were detected by western blotting using specific antibodies in mice brain. β-Actin protein was used as an internal control. Each blot is representative for three experiments. The values in the western blot band indicate average density over β-actin from 5 animals. #, Significantly different from control group (p < 0.05). *, Significantly different from LPS-treated group (p < 0.05). Control, saline-treated group. LPS, lipopolysaccharide. MH, 4-O-methylhonokiol.