Effect of 4-
-methylhonokiol on the LPS-induced neuroinflammation. (A) Immunoreactive cells of anti-GFAP antibody were investigated in the cortex and hippocampus. (B) Immunoreactive cells of anti-Iba1 antibody were investigated in the cortex and hippocampus. The present figure is representative for three different experiments with different animal brains. (C) The level of GFAP and Iba1 was detected by western blotting using specific antibodies in mice brain. β-Actin protein was used as an internal control. Each blot is representative for 3 experiments. The values in the western blot band indicate average density over actin from 5 animals. (D) Co-immunoreactivity against anti-GFAP antibody (red label) and anti-Aβ antibody (green label) was investigated. The figure representative of three experiments from different mice brain. #, Significantly different from control group (p < 0.05). *, Significantly different from LPS-treated group (p < 0.05). Control, saline-treated group. LPS, lipopolysaccharide. MH, 4-O-methylhonokiol.