Effect of 4-
-methylhonokiol on the LPS-induced neuronal cellular damage. (A) Immunoreactive cells of anti-cleaved caspase-3 antibody were investigated in the cortex and hippocampus. The present figure is representative for 3 different experiments with different animal brains. (B) Apoptotic cell death was determined by DAPI staining and TUNEL assay as described in Methods. Each panel represents 6 animals. (C) The levels of cleaved caspase-3 and caspase-3 were detected by western blotting using specific antibodies in mice brain. β-Actin protein was used as an internal control. The values in the western blot band indicate average density over β-actin from 5 animals. #, Significantly different from control group (p < 0.05). *, Significantly different from LPS-treated group (p < 0.05). Control, saline-treated group. LPS, lipopolysaccharide. MH, 4-O-methylhonokiol.