NOV expression in DRG and dorsal horn of spinal cord (DHSC) during CFA-induced pain. (A, B) NOV mRNA expression in DRG (A) and DHSC (B) ipsilateral to CFA injection in the hind paw. Transcript levels were quantified by RT-qPCR. Values were normalized to rat S26 mRNA levels. The corresponding control of the same time course (sham, sh) was set to 1. Data represent the mean value ± SEM of two independent experiments realized with four rats per condition. (**P < 0.01 CFA versus corresponding sham). (C) Expression of NOV protein in the DHSC. Representative western blot (left panel) and quantification of the protein levels normalized to GAPDH (right panel). Values are reported relative to the corresponding control (sham, sh) of the same time course (set to 1). Data represent the mean value ± SEM of two independent experiments realized with three rats per condition (*P < 0.05, CFA versus corresponding sham).