Expression of r
C1q in the
yeast host system. (A) Immunodetection of rHmC1q (selected positive clone) by western blot with the polyclonal anti-HmC1q antibodies is compared with the native HmC1q released in leech central nervous system (CNS)-conditioned medium (control). Supernatant from non-transformed yeast culture was used as a negative control. (B) Part of the chromatogram of the yeast culture illustrating the purification of rHmC1q by reverse-phase high performance liquid chromatography (RP-HPLC). The rHmC1q molecule was eluted at 10% of acetonitrile (see arrow) and was detected by western blot with the polyclonal anti-HmC1q antibody.