Figure 10

Diphenyleneiodonium (DPI) inhibited ethanol-induced microglial activation. Male C57BL/6 mice were treated intragastrically with water or ethanol (5 g/kg, i.g.) daily for 10 days. Diphenyleneiodonium (DPI) (3 mg/kg) was injected intraperitoneally 0.5 hr and 24 hr after the last dose of ethanol. Mice were sacrificed 3 hrs after the last dose of DPI. Brain sections were stained with Iba-1 antibody. In water control mice, most of the microglia were in a resting morphological shape. Iba1+IR cells in ethanol-treated mouse brains were activated as shown by increased cell size, irregular shape, and intensified Iba1 staining. DPI inhibited ethanol-induced microglial activation shown in a resting morphological shape. Scale bar = 200 μm.