Figure 11
DPI inhibited ethanol-induced production of O 2 - and O 2 - -derived oxidants. C57BL/6 mice treated with water, ethanol, DPI and ethanol plus DPI were injected with dehydroethidium (10 mg/kg, i.p.) 2.5 hrs after the last dose of DPI. Brains were harvested 30 min later and frozen sections (15 μm) were examined for hydroethidine oxidation product, ethidium accumulation, by fluorescent microscopy. (A) The images demonstrated that ethanol markedly increased production of O2 - and O2 --derived oxidants compared to water controls, and DPI significantly reduced ethanol-induced production of O2 - and O2 --derived oxidants compared to ethanol-treated group. Scale bar = 200 μm. (B) Level of fluorescent intensity of ethidium positive cells in cortex was quantified by BioQuant image analysis software. Ethanol treatment of C57BL/6 mice showed significantly increased O2 - and O2 --derived oxidant production, compared with water control mice. DPI significantly decreased ethanol-induced increases in the fluorescent intensity of O2 - and O2 --derived oxidant production. ** P < 0.01, compared with water controls. ## P < 0.01, compared with ethanol-treated group.