Figure 6

Chronic ethanol up-regulates brain NOX subunit gp91 ^phox expression. Brain sections from C57BL/6 mice treated with water or ethanol (5 g/kg, i.g.) daily for 10 days and sacrificed 24 hrs and 1 week after ethanol exposure were stained with monoclonal mouse gp91^phox antibody. Immunolabeling was visualized by using nickel-enhanced 3,3'-diaminobenzidinne (DAB) for 24 h samples and Alexa Fluor 488 dye (green) for 1 week samples. (A) The images shown are representative of gp91^phox +IR cells in cortex and DG 24 hrs after water and ethanol exposure. Scale bar = 200 μm. (B) Chronic ethanol significantly increased the level of gp91^phox immunoreactivity 24 hrs after ethanol treatment, as quantified by BioQuant image analysis system. (C) Chronic ethanol-induced increase in gp91^phox expression remained elevated at 1 week after ethanol treatment. Scale bar = 100 μm. ** P < 0.01, compared with the water control mice.