Figure 2
From: Therapeutic targeting of Krüppel-like factor 4 abrogates microglial activation
Effect of honokiol on reactive oxygen species and nitric oxide generation in lipopolysaccharide-stimulated BV-2 cells. Total cellular extract and supernatant detected for the levels of ROS and NO in LPS- and HNK-treated cells. Representative FACS plot showing intracellular ROS production. (A) Increase in the levels of ROS generation upon LPS treatment with respect to untreated control condition. (B, C) Decrease in ROS generation upon treatment with 1 μM and 10 μM HNK in LPS-stimulated cells. (D) Graph representing the MFI of ROS generation. (E) Graph representing nitrite concentration in LPS and LPS + HNK conditions. Nitrite is measured in μM quantities. Data represent mean ± SEM from three independent experiments performed in duplicate. *P < 0.05 and **P < 0.01 in comparison to LPS-treated values.