Pathology spinal cords. Upper panel: viable cells. Pathology is characterized by marked subpial demyelination (A, B, Luxol fast blue). The rectangle in (A) marks the region enlarged in (B-D). The demyelinated areas show some activated microglia and macrophages (C, Mac-3) as well as moderate PrPSc deposition, which is most abundant at the lesion border (D, arrowheads, PrPSc). Lower panel: homogenated cells. In contrast, control animals were devoid of demyelination (E, F, luxol fast blue). The rectangle in (E) marks the region enlarged in (F-H). White matter only reveals some activated microglia (G, Mac-3) but no PrPSc deposition (H).