Figure 2From: Peroxisome deficiency but not the defect in ether lipid synthesis causes activation of the innate immune system and axonal loss in the central nervous system Myelin loss and mild microgliosis in the cortex. (A-D) Double immunolabeling of myelin (MBP, red) and activated microglia (MAC-3, green) or microglia (F4/80, green), respectively. Cortical demyelination was detected in a subset of Nestin-Pex5 −/− mice at six weeks (B and D), but a strong microglial reaction was never seen (B and D). However, the observed microglial cells were MAC-3 positive, which indicates phagocytotic activity (B) and some microglia seem to contain myelin (D, arrow). (E-F) Loss of axons, represented as a decreased SMI31 immunoreactivity, was clearly detectable in the cortex of 12-week-old knockout mice (F), when compared to the control mice (E). All panels of each row were stained with the same antibodies. Scale bars: A-C and G-I: 100 μm; D-F: 25 μm. MBP, myelin basic protein.Back to article page