Effects of FK866 on Bcl-2 expression. Moreover, a basal level of Bcl-2 expression was detected in spinal cord from sham-operated mice (a, a1 and b). Twenty-four hours after SCI, Bcl-2 expression was significantly reduced in spinal cord from SCI mice (a, a1 and c). Treatment with FK866 (a, a1 and d) significantly reduced the SCI-induced inhibition of Bcl-2 expression. The relative expression of the protein bands was standardized for densitometric analysis to β-actin levels, reported in panel a, a1 and are expressed as mean ± S.E.M from n = 5/6 spinal cord for each group. *p < 0.01 vs sham, °°p < 0.001 vs SCI + vehicle.