Figure 4

Dexamethasone reduces levels of CCL2, IL-6, and IL-8 induced by B. burgdorferi in differentiated oligodendrocytes. Evaluation by the multiplex ELISA assay of culture supernatants of differentiated MO3.13 oligodendrocytes incubated with live B. burgdorferi spirochetes at a multiplicity of infection (MOI) of 10:1 for 48 h in the presence and absence of dexamethasone (Dex) at 5 μM, 15 μM, and 150 μM concentrations and carrier substance (2-hydroxypropyl)-β-cyclodextrin (HPC), at 15 μM, 45 μM, and 450 μM, respectively, showing levels of CCL2 (A), IL-6 (B), and IL-8 (C). Levels of CCL2 (D) and IL-8 (E) detected in culture supernatants of differentiated primary human oligodendrocytes, as induced by live B. burgdorferi after 48 h of incubation in the presence and absence of dexamethasone at 5 μM, 15 μM, and 150 μM concentrations (*P < 0.05).