PrP106-126 induces caspase-1 activation. Cells were primed with 300 ng/ml lipopolysaccharide (LPS) for 3 hours and then left untreated or stimulated with PrP106-126 (100 μmol/l) or Scr (100 μmol/l). Data show western blot analysis of caspase-1 (p20) cleavage in (a) LPS-primed BV2 and (b) the ANA1 cell line. p45, full-length pro-form of caspase-1; p20, active subunit of caspase-1. Data represent one of three independent experiments.