Figure 5
From: The NALP3 inflammasome is involved in neurotoxic prion peptide-induced microglial activation
High concentration of K + and N-acetyl-cysteine (NAC) blocks PrP106-126-stimulated release of interleukin (IL)-1β in BV2 microglia. Lipopolysaccharide (LPS)-primed BV2 microglia were treated with PrP106-126 in combination with either K + or NAC for 6 hours, then the supernatants were collected for (a) ELISA analysis of the secreted IL-1β, and (b) qPCR analysis of the mRNA expression of NALP3 and ASC). Data were performed in triplicate and expressed as the mean ± SD, and are representative of three separate experiments. *P < 0.05, significantly different from PrP106-126 treated cells.