Nuclear factor (NF)-κB activation is required for NACHT, LRR and PYD domains-containing protein (NALP)3-ASC inflammasome activation in PrP106-126-stimulated microglia.
(a) BV2 cells were stimulated with PrP106-126 (100 μmol/l) for 6, 12 and 24 hours, then the cytoplasmic protein and nuclear protein were collected and analyzed for NF-κB p65 translocation by western blot analysis. (b) BV2 cells stimulated with PrP106-126 were pretreated with Bay11-7082, a specific inhibitor of NF-κB (10, 15, and 20 μmol/l) or PBS (as a negative control) for 1 hour, and the mRNA expression of NALP3 and ASC was determined by quantitative PCR. Data represent the mean ± SD of triplicate samples from one of three independent experiments. *P < 0.05, significantly different from PrP106-126 treated cells.