Table 1 Unpredictable chronic mild stress procedure
Week | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | Sunday |
|---|---|---|---|---|---|---|---|
First week | 8:00 AM to 10:00 PM: tilted cage | 10:00 AM to 12:00 AM: cage shaking for 10 minutes | 17:00 PM to next day: damp bedding | 9:00 AM to 11:00 AM: swimming at 4°C for 5 minutes | 15:30 PM to 17:30 PM: confinement in tube for 2 h | 22:00 PM to next day: food and water deprivation | 21:00 PM to 22:00 PM: sucrose preference test |
Second week | 17:30 PM to next day: persistent illumination | 9:00 AM to 11:00 AM: 45°C oven for 5 minutes | 18:00 PM to next day: tilted cage | 13:30 PM to 15:30 PM: confinement in tube for 2 h | 19:00 PM to next day: damp bedding | 20:00 PM to next day: food and water deprivation | 19:30 PM to 20:30 PM: sucrose preference test |
Third week | 10:30 AM to 12:30 PM: confinement in tube for 2 h | 17:00 PM to next day: damp bedding | 8:00 AM to 10:00 PM: tilted cage | 19:30 PM to next day: persistent illumination | 10:00 AM to 12:00 AM: cage shaking for 10 minutes | 21:00 PM to next day: food and water deprivation | 20:00 PM to 21:00 PM: sucrose preference test |
Fourth week | 19:00 PM to 21:00 PM: 45° C oven for 5 minutes | 8:00 AM to 10:00 PM: tilted cage | 13:30 PM to 15:30 PM: confinement in tube for 2 h | 9:00 AM to 11:00 AM: cage shaking for 10 minutes | 17:30 PM to next day: persistent illumination | 23:00 PM to next day: food and water deprivation | 22:30 PM to 23:30 PM: sucrose preference test |