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Table 1 Unpredictable chronic mild stress procedure

From: Inducible nitric oxide synthase is involved in the modulation of depressive behaviors induced by unpredictable chronic mild stress

Week Monday Tuesday Wednesday Thursday Friday Saturday Sunday
First week 8:00 AM to 10:00 PM: tilted cage 10:00 AM to 12:00 AM: cage shaking for 10 minutes 17:00 PM to next day: damp bedding 9:00 AM to 11:00 AM: swimming at 4°C for 5 minutes 15:30 PM to 17:30 PM: confinement in tube for 2 h 22:00 PM to next day: food and water deprivation 21:00 PM to 22:00 PM: sucrose preference test
Second week 17:30 PM to next day: persistent illumination 9:00 AM to 11:00 AM: 45°C oven for 5 minutes 18:00 PM to next day: tilted cage 13:30 PM to 15:30 PM: confinement in tube for 2 h 19:00 PM to next day: damp bedding 20:00 PM to next day: food and water deprivation 19:30 PM to 20:30 PM: sucrose preference test
Third week 10:30 AM to 12:30 PM: confinement in tube for 2 h 17:00 PM to next day: damp bedding 8:00 AM to 10:00 PM: tilted cage 19:30 PM to next day: persistent illumination 10:00 AM to 12:00 AM: cage shaking for 10 minutes 21:00 PM to next day: food and water deprivation 20:00 PM to 21:00 PM: sucrose preference test
Fourth week 19:00 PM to 21:00 PM: 45° C oven for 5 minutes 8:00 AM to 10:00 PM: tilted cage 13:30 PM to 15:30 PM: confinement in tube for 2 h 9:00 AM to 11:00 AM: cage shaking for 10 minutes 17:30 PM to next day: persistent illumination 23:00 PM to next day: food and water deprivation 22:30 PM to 23:30 PM: sucrose preference test