Figure 4From: Lipopolysaccharide-induced neuroinflammation leads to the accumulation of ubiquitinated proteins and increases susceptibility to neurodegeneration induced by proteasome inhibition in rat hippocampus Proteasome inhibition after intrahippocampal injection of LPS induced the formation of aggresome-like structures in hippocampal neurons. (A) Upper: immunofluorescence analysis of the cellular distribution of ubiquitinated proteins in hippocampal slices from rats treated with saline, saline + LPS, saline + LT and LPS + LT. Images correspond to animals sacrificed 48 hours after the last injection. Lower: higher magnification corresponding to neurons from the stratum pyramidale taken from images showed above. Nuclei were counterstained with DAPI. Note the presence of large aggresome-like structures (arrowheads) only in the LPS + LT-injected rats. Bar scale: 75 μm for upper images and 10 μm for lower images. (B) Similar as in A but images correspond to aged rat hippocampus injected with LT alone. DAPI: 4′-6-diamidino-2-phenylindole; LPS: lipopolysaccharide; LT: lactacystin; sp: stratum pyramidale.Back to article page