Figure 1
Schematic and timeline for chronic stress. Mice were subjected to PS, CUS, or no stress (controls) for 28 consecutive days. To determine whether chronic stress resulted in habituation of the plasma CORT response, blood was collected from the facial vein 2 weeks prior to the first day of stress (Day -14), and 15 min after stress on Day 28. For non-stressed control mice, blood was taken on same days as above. The marble burying, sucrose preference, and tail suspension test were conducted 7-10 days following the final day of stress. To determine whether chronic stress modulated the inflammatory response to a subsequent challenge, mice were injected with 7.5 × 105 EU/kg LPS at Day 42 and tissue was processed for analysis of gene expression.