Figure 5

Retinal whole-mount. Double immunostaining for NF-200 and GFAP after 15 days of laser-induced OHT. Axon and RGCs (in red). Macroglia (in green). A-C: Images correspond to area 1 of study. D: image corresponds to area 3 of the study. A-B: NF-200 labelling of RGC axons was rectilinear and uniform in naïve (A) and contralateral eyes (B). NF-200+RGC somas were infrequently observed; C-D: beads on a string-like immunostaining (arrowhead) and small varicosities (yellow arrow) in NF-200+ axons in OHT-eyes. Abnormal staining of cell bodies and primary dendrites of RGCs (white arrow) were more frequently found in OHT-eyes (D) than in naïve and contralateral eyes. Confocal microscopy. GFAP, glial fibrillary acidic protein; OHT, ocular hypertension; RGCs, retinal ganglion cells.