Retinal whole-mount. Double immunostaining for GFAP (red) and MHC-II (in green) after 15 days of laser-induced OHT. A-C: naïve eyes; D-F: contralateral eyes; G-I: OHT-eyes. In contralateral eyes MHC-II immunoreaction of astrocytes (arrow) and Müller cells (arrowhead) in (E) was increased with respect to naïve eyes (arrow) in (B). In OHT-eyes, MHC-II immunoreaction of Müller cells (arrowhead) in (H) was notably upregulated in comparison with contralateral (E). Fluorescence microscopy and image acquisition using the ApoTome. GFAP, glial fibrillary acidic protein; MHC, major histocompatibility complex; OHT, ocular hypertension.