Primary brain vascular pericytes. Phase contrast images of: (A) an uninfected subconfluent monolayer of primary brain vascular pericytes, (B) a confluent monolayer of brain vascular pericytes, and (C) pericytes 72 hours after infection with SBCMV. Immunofluorescence staining of SBCMV-infected pericytes for (D) HCMV MIE protein and (E) pp65 late protein. (F) TEM of SBCMV-infected pericytes showing HCMV virions in the cytoplasm (see arrow). With the exception of the TEM, images were taken on a Nikon TE2000S microscope (200x magnification). HCMV, human cytomegalovirus; MIE, major immediate early protein; SBCMV, primary HCMV isolate from a patient; TEM, transmission electron microscopy.