Expression of SBCMV pp65 virion protein mRNA by pericytes, BMVEC and astrocytes. Real-time PCR analysis of pp65 late virion protein mRNA levels in SBCMV-infected pericytes, BMVEC and astrocytes. cDNA was prepared from total RNA isolated at 24 and 96 hours postinfection along with RNA from mock-infected control cells. Black bars indicate fold transcription for SBCMV-infected pericytes, BMVEC and astrocytes. This experiment was replicated three times and normalized to GAPDH. BMVEC, brain microvascular endothelial cells; GAPDH,gyceralaldehyde phosphate dehydrogenase; pp65, human cytomegalovirus phosphorylated envelop protein expressed at late times during virus replication; PCR, polymerase chain reaction; SBCMV, primary HCMV isolate from a patient.