Table 1 Primary and secondary antibodies used in immunocytochemistry and western blotting
From: Microglia P2Y6 receptors mediate nitric oxide release and astrocyte apoptosis
Primary antibodies | ||||
|---|---|---|---|---|
Antigen | Code | Host | Dilution | Supplier |
GFAP | G9269 | Rabbit | 1:600 (IF) | Sigma |
GFAP | G6171 | Mouse | 1:600 (IF) | Sigma |
CD11b | sc-53086 | Mouse | 1:50 (IF) | Santa Cruz Biotechnology, Inc |
P2Y6 | APR-011 | Rabbit | 1:200 (IF) | Alomone |
1:300 (WB) | ||||
iNOS | AB5382 | Rabbit | 1:5 000 (IF) | Chemicon |
Actin | sc-1615-R | Rabbit | 1:200 (WB) | Santa Cruz Biotechnology, Inc |
Secondary antibodies | ||||
Antigen | Code | Host | Dilution | Supplier |
TRITC anti-rabbit | T6778 | Goat | 1:400 (IF; GFAP, P2Y6) | Sigma |
1:2 000 (IF; iNOS) | ||||
Alexa Fluor 488 anti-mouse | A-11034 | Goat | 1:400 (IF) | Mol. Probes |
anti-rabbit conjugated to horseradish peroxidase | sc-2004 | Goat | 1:10 000 (WB) | Santa Cruz Biotechnology, Inc |