Figure 2

Attenuated rabies virus (RABV) infection increases infiltration of immune cells into the central nervous system (CNS). Suckling mice were intracerebrally (i.c.) infected with aG and CTN, and brains and spleens were harvested on day 7 postinfection (p.i.) (n ≥3, mean ± SD). The percentages of CD11b^hi F4/80⁺ cells (A), CD3⁺, CD4⁺ and CD8⁺ T lymphocytes (C) from brain infiltration of lymphocytes (BILs) were measured by flow cytometry. (B) Suckling mice were i.c. infected with CTN (5.6 × 10³ FFU) or PBS and then intraperitoneally (i.p.) injected with BrdU on day 5 p.i.. BILs in the brain were isolated and subjected to flow cytometry analysis. (D) Number of CD3⁺ T cells infiltrated into different regions of the brain, including cerebral cortex, hippocampus, thalamus and hypothalamus, were quantified by immunohistochemistry (IHC) staining. Data were presented as means ± SD (**P <0.01; ***P <0.001).