Figure 5

Effect of tranexamic acid on plasminogen transfer through the blood-cerebrospinal fluid barrier and the renal glomerulus. Rats with lipopolysaccharide (LPS)-induced systemic inflammation received an intravenous injection of plasminogen labelled with Alexa Fluor 488 dye (A488-Pg) alone (group L2) or in the presence of sustained concentrations of tranexamic acid (TXA) (group L3) (see flowchart in Figure 1). Plasminogen was detected by immunoblotting. (A) Representative immunoblot of rat plasminogen (Pg), plasma diluted 1:50 cerebrospinal fluid (CSF) and urine samples using a rabbit antibody to mouse plasminogen. Experiments were performed as indicated in Figure 2 with an equal volume (10 μl) of each sample. (B) Representative column bars of plasminogen in plasma, CSF and urine after immunoblot and densitometric analysis.