Figure 2
From: Diet-induced obesity and low testosterone increase neuroinflammation and impair neural function
Low testosterone and high-fat diet increase proinflammatory cytokines in cortex. (A) Representative agarose gel of RT-PCR products shows the relative levels of TNFΑ, IL-1Β, and β-actin mRNAs in sham GDX (Sham), GDX, and GDX mice with testosterone treatment (GDX + T) in both control-diet and high-fat diet. (B) Quantitative real-time PCR data show the mean (±SEM) expression levels compared to the Sham control group for TNFΑ mRNA. (C) Quantitative real-time PCR data show the mean (±SEM) expression levels compared to the Sham control group for IL-1Β mRNA. All data are normalized with corresponding β-actin values. Statistical significance is based on ANOVA followed by Bonferroni. * P ≤0.05 between diet groups; ** P ≤0.05 between hormone groups; N ≥6.