Figure 4

High-fat diet derived glia cultures support reduced neuron viability. Primary cortical neurons were plated either by themselves or on confluent primary glial cultures derived from the cortices of sham GDX (Sham), GDX, and GDX mice with testosterone treatment (GDX + T) in both control-diet and high-fat diet. Top panels show representative pictures of neurons co-cultured with control diet (A) and high-fat diet (C) derived glia. (B) Quantitative graph for percentage of neurons per treatment group in the neuron-glia co-culture. (D) Quantitative graph for percent neuron viability in conditioned glial media from each group. Data show mean cell viability (±SEM) of a representative experiment. * P ≤0.05 between diet groups; ** P ≤0.05 between hormone groups; N = 3.