Figure 1
α 1 -antitrypsin (A1AT) reduces lipopolysaccharide (LPS)-induced release of pro-inflammatory mediators. A-C. Cell culture supernatants of LPS and/or A1AT co-treated cells were subjected to ELISA to detect the levels of pro-inflammatory cytokines. Therefore, primary microglial cells were treated with 0.1 μg/ml LPS and/or 2 and 4 mg/ml A1AT for 24 hours, followed by quantification of TNF-α, IL-6, and IL-1β levels. Values are given in ng/ml. Means and standard deviations of the mean of three independent experiments are shown (*P value ≤ 0.05, **P value ≤ 0.01, n.s., not significant). D. Nitric oxide (NO) was measured in the same cell culture supernatants using Griess reagents. Values are given in μM. Means and standard deviations of the mean of three independent experiments are shown (*P value ≤ 0.05, **P value ≤ 0.01).