Figure 9

Immunoexpression of NKCC1 (red) and NeuN (green) in different layers of the olfactory bulb (A-F). Scale bar =20 μm (A-F). NO content (μM) and glutamate concentration (mg/ml) analysis (G,H) in the olfactory bulb of the C group, the SI + S group (0.5, 3, 24, and 72 h) and the SI + AG or SI + L-NAME groups (3, 24, and 72 h) compared with the C group. (* P <0.05; ** P <0.01). (A-F) Colocalized expression of NKCC1 with NeuN immunoreactive neurons (arrows) is detected in the C group. Note that a large number of NeuN-positive neurons are double labeled with NKCC1 (F) compared with the C group (C). (G) [NO]/μM is significantly increased in the olfactory bulb in SI + S group at 0.5, 3, 24, and 72 h, as compared with the C group; however, [NO] is significantly suppressed in SI + AG group at 24 and 72 h, as compared with saline administration; [NO] is not significantly suppressed at 24 and 72 h in the SI + L-NAME group, as compared with the SI + S group. (H) Glutamate concentration is significantly increased in the olfactory bulb in the SI + S group at 3, 24, and 72 h, as compared with that of the C group; it is not significantly suppressed in the SI + AG group at 24 and 72 h, as compared with the SI + S group.