Figure 2From: Partial suppression of M1 microglia by Janus kinase 2 inhibitor does not protect against neurodegeneration in animal models of amyotrophic lateral sclerosis Pharmacological properties of R723 and its effects on peripheral monocytes. (A, B) Pharmacological profile of R723 in plasma and spinal cord tissue after single-dose administration by oral gavage to 120-day-old female mSOD1G93A mice. Concentration of the compound at the indicated time points was quantified by LC/MS/MS assay. (C, D) Effects of R723 treatment on the proportion of CD11b + or Ly6c + cells in peripheral blood. mSOD1G93A mice (120 days old) were orally administered with vehicle or R723 (70 mg/kg) for 4 days. Blood were harvested, and the proportions of CD11b + and Ly6c + cells were evaluated by FACS. (C) The proportion of CD11b + monocytes was significantly reduced in peripheral blood of R723-treated mSOD1G93A mice. (D) Four-day treatment with R723 reduced the proportion of Ly6c + CD11b + monocytes in peripheral blood. In panels (C) and (D), n = 3 for vehicle-treated mice, and n = 4 for R723 treated mice. Data are expressed as means ± SEM. *P < 0.05, Mann-Whitney U-test.Back to article page