Cytokine profiles by peripheral blood monocytes are associated with changes in behavioral symptoms following immune insults in a subset of ASD subjects: an inflammatory subtype?

Table 6 IL-10 production by purified peripheral blood monocytes in the study groups

The study group	Without stimuli [pg/ml]	LPS ratio^a	Zymosan	CL097
ASD-IS^c
- Flare	848.6 ± 344.8	1.48 ± 0.88^b	1.15 ± 0.43^b	0.55 ± 0.63²
- Non-Flare	648.7 ± 382.1	2.64 ± 1.37	2.15 ± 1.09	1.23 ± 1.12
ASD/NFA	812.4 ± 365.5	1.96 ± 0.91	1.55 ± 0.75	0.89 ± 0.72
ASD/non-NFA	855.4 ± 364.7	1.93 ± 1.12	1.63 ± 1.03	0.93 ± 0.91
Non-ASD/NFA	948.5 ± 362.7	1.93 ± 1.38	1.66 ± 1.67	0.67 ± 0.51
Normal control	677.7 ± 290.0	2.40 ± 1.10	1.93 ± 1.06	0.76 ± 0.69
PANS
- Flare	791.7 ± 449.4	1.87 ± 0.95	1.82 ± 1.27	1.38 ± 0.99
- Non-Flare	945.8 ± 455.4	1.93 ± 1.14	1.66 ± 0.84	1.58 ± 1.07

^aIL-10 production in response to LPS, zymosan, and CL097 were expressed as a ratio of IL-10 produced in these stimulants/IL-10 produced in the absence of stimuli. It should be noted that IL-10 production tends to be lower in the presence of CL097 in most of the study groups.
^bIL-10 production by `flare’ ASD-IS cells were lower than `non-flare’ ASD-IS cells with matched pair analysis as detailed in Figure IL-10 production by `flare’ ASD-IS cells in response to LPS and zymosan were also lower than ASD/NFA and normal control cells (P <0.01). IL-10 production by `flare’ ASD-IS cells in response to CL097 cells were also lower than those produced by PANS cells (P <0.01).
^c Abbreviations used; ASD, autism spectrum disorder; ASD-IS, ASD-inflammatory subtype; IL, interleukin; LPS, lipopolysaccharide; NFA, non-IgE mediated food allergy; PANS, pediatric acute-onset neuropsychiatric syndrome.

ISSN: 1742-2094